Detection of Mycoplasma gallisepticum using Polymerase Chain Reaction(PCR)
1999
Lee, Y.J. | Kim, K.S. | Kim, J.W. (Ministry of Agriculture and Forest, Anyang (Korea Republic). National Veterinary Research and Quarantine Service) | Tak, R.B. (Kyungpook National University, Taegu (Korea Republic). College of Veterinary Medicine)
A species-specific 760 base pair(bp) BamHI to EcoRI DNA fragment(fMG-2) of lipoprotein gene was isolated from a Mycoplasma gallisepticum (M gallisepticum) genomic library. Based on the DNA sequence data of fMG-2, a pair of 25bp primers was synthesized. When used in the polymerase chain reaction(PCR), 732bp DNA products were amplified from 6 standard strains and 10 field isolates of M gallisepticum, but not from 2 Mycoplasma synoviae and 7 other Mycoplasma species. The lower detection limit was 100fg of the genomic DNA. Identity of the PCR products was confirmed by comparison of patterns of restriction endonuclease analysis with AseI, DraI, EcoRV and SspI.
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