Plant regeneration and in vitro propagation from several tissue explants of Solanum integrifolium Poir
1995
Iwamoto, Y. (Osaka-fu. Agricultural and Forestry Research Center, Habikino (Japan)) | Kagi, T.
Greater parts of rootstock for aubergine culture in Japan are Solanum integrifolium Poir., because it has completely resistance to Fusarium wilt and the grafting aubergines get higher yields than the plants of scion root. However, Verticillium wilt and bacterial wilt diseases induce the deteriorations of yields and fruit quality in recent years. It is hoped that an introduction of the resistance traits to S. integrifolium will succeed by biotechnological methods (e.g. cell selection, cell fusion and genetic transformation). The purpose of this study is to develop the efficient regeneration system which is a fundamental technique for these methods. Shoot formation from hypocotyl explant was established on Murashige and Skoog's solid medium supplemented with 1% sucrose and 0.2 mg/l Zeatin. After 4 week culture, 24.9 shoots per explant were obtained from all hypocotyl sections. Shoot organogenesis was induced on the medium supplemented with Zeatin from all kinds of explants (e.g. hypocotyl, cotyledon, leaf, stem internode and petiole). However, the most suitable concentrations of Zeatin and the number of formatting shoots were different in kind of explants. Hypocotyl, stem internode and petiole explants were suitable for in vitro propagation of S. integrifolium. Rooting from shoots was established on 1/2 MS solid medium without hormone. All regenerated plants grew vigorously and bore their fruits with seeds in greenhouse. In conclusion, this regeneration system and in vitro propagation technique of the plant were established
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