Isolation, characterization, identification and antimicrobial assay of the active components of kakawate [Gliricidia sepium] leaves
2000
Rabena, A. (University of Northern Philippines, Vigan, Ilocos Sur (Philippines))
The active component of kakawate leaves was isolated and made it a suitable pesticide. Kakawate leaves are macerated and soaked overnight. Using petroleum ether as solvent , percentage recovery yielded 5.35% of the total fresh weight. Findings showed that column chromatography of crude petroleum ether extract yielded two pure compounds, MWF1 and PEF2. While MWF1 was not effective in killing termites, PEF proved to be 100% effective. Bioassay of pure PEF2 compound at 0.02 g/mL caused 95% termite mortality 4 hours after feeding and 100% mortality after 8 hours. Further tests confirmed that PEF2 is cyclic ester, a lactone named coumarin, with the structure - C9H6O2. Coumarins isolated from kakawate leaves using petroleum ether show bioactivity. Coumarins inhibited the growth of fungus Tricophyton mentagrophytes. The cost of extracting coumarins from kakawate is P31.10/g. This was actually more expensive than synthesizing the compound in the laboratory which only costs P24.50/gram
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