Studies on increasing the yield of in vitro embryo production in water buffaloes
1999
Duran, D.H. | Gunjima, M. | Pedro, P.B. | Aquino, F.P. | de Vera, R. | Duran, P.G. | Cruz, L.C. (Philippine Carabao Center, Munoz, Nueva Ecija (Philippines). Embryo Biotechnology Lab.)
Results revealed that significantly higher number of oocytes could be retrieve from the ovaries using the slicing method than aspiration method (7.33 vs. 3.54 oocytes/ovary, respectively). Similarly, higher number of good oocytes could be retrieve in using slicing method than in aspiration method (1.34 plus or 4minus 0.50 and 1.83 plus or minus 0.25 vs. 0.43 plus or minus 0.15 and 0.92 plus or minus 0.28 Rank A and B oocytes, respectively). When in vitro development potential of the classified oocytes were assessed, significantly higher (P0.01) percentage of the Rank A oocytes cleaved and developed to the blastocyst stage than in the other counterparts (56.86% and 25.49% against 30.00% and 2.00% for Rank B and 8.82% and O for Rank C and O for Rank D and E, respectively). Comparing the efficiency of three commercially prepared in vitro culture media, results showed that TCM-199 is the most efficient basic medium for buffalo in vitro embryo production (59.46% and 24.32% cleavage and blastocyst development rate vs. 23.53% and 5.88% for MEM and 16.30% and 2.17% for IVMD101, respectively). However, difference on the efficiency of different lot numbers in TCM-199 medium was observed. These results suggests that there are so many factors to consider for efficient in vitro embryo production. Further studies to improve the system are therefore recommended
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