RAPD markers linked to major genes behind field resistance against the green spruce aphid Elatobium abietinum (Walker) in Picea sitchensis (Bong. (Carr.))
2000
Skov, E. | Wellendorf, H. (Royal Veterinary and Agricultural Univ., Horsholm (Denmark). Arboretum)
Within the open-pollinated offspring of two orchard clones which have been heavily attacked by the green spruce aphid in 1989, a large number of dominant RAPD-markers has been screened for co-segregation with recorded field resistance. Three of these markers - one in the first family and two in the second family co-segregated. In the first family further checks of the zygosity of the mother performed on her haploid megagametophytes confirmed her heterozygosity at the marker locus. The allele frequency amongst the remaining orchard clones where unity for the recessive allele, i.e. a near-perfect test-cross situation was revealed for this particular marker. In the second family the two markers co-segregating with resistance segregated independent of each other. A highly significant interaction between these two markers concerning field resistance indicates epistatic gene effects between two nonlinked resistance genes. Considering this evidence it is concluded, that three RAPD-markers may have been identified, each of which is linked to segregating resistance gene-loci coding for aphid resistance in the offspring from these two particular parent clones. The total number of detected resistance gene-loci may be two, possibly three. Average effects of each combination of the resistant gene and linked marker covered a range of 0.6-1.2 times the within family phenotypic standard deviation in field resistance
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