Effect of pleuran on colitis in rat
2001
Bobek, P. | Cerna, S. (Ustav Preventivnej a Klinickej Mediciny, Bratislava (Slovak Republic)) | Nosalova, V.
The effects of pleuran, a beta-glucan isolated from Pleurotus oestratus, were studied in a model of acute colitis in rats. Pleuran was given either as a 2% food component or as a 0.44% pleuran hydrogel drink over 4 weeks. Colitis was induced by intraluminal instillation of 4% acetic acid and, after 48 hours, the animals were killed/scarified and the extent of colonic damage and several biochemical parameters were examined. The macroscopic damage score was reduced by 51% or 67% by pleuran diet and pleuran hydrogel drink, respectively. At both regimens, pleuran prevented significant colonic wet weight increase which was observed in the control diet group. The enhaced activity of myeloperoxidase in the inflamed colonic segment was reduced by both pleuran diets. In the control untreated group the colonic damage was accompained by decreased activities of lysosomal enzymes, acid phosphatase and cathepsin D, whereas at both pleuran regimens the decrease was significantly attenuated. Both pleuran regimens reduced the content of conjugated dienes in the erythrocytes, liver and colonic tissue. In contrast to this fact, activities of antioxidant enzymes were not so greatly influenced. Significant increase was found in the case of superoxide dismutase activity in sham operated rat erythrocytes under influence of both pleuran regimens. In animals with induced colitis, the activity of superoxide dismutase in colon and glutathione transferase in erytrocytes increased under the influence of pleuran hydrogel. Our results indicate the simulation of antioxidant defence takes part in protective effect of dietary pleuran
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