[Use of phyllosphere and rhizosphere microflora in biological control of peanut black leaf spot disease (Phaeoisariopsis personata)]
1993
Tharmmasak Sommartya | Nongnut Kiwacha (Kasetsart Univ., Bangkok (Thailand). Faculty of Agriculture. Dept. of Plant Pathology)
Phyllosphere and rhizosphere microflora of peanut were isolated from plant and soil specimens obtained from three peanut planting areas (viz. National Corn and Sorghum Research Centre, NCSRC, Nakhon Ratchasima: Kampangsaen and Bangkhen Campuses of Kasetsart University). Techniques for isolating these microflora were spore fall method, leaf imprint method and leaf washing method. Among these technique, the leaf imprint method yielded maximum number of microorganisms. Besides, most of microorganisms were obtained from upper surface of peanut leaf. Effectiveness of these microflora as a biological control agents (BCA) was tested by applying the spore suspension on to peanut leaves in the field and in laboratory (detached leaf technique). Significant reduction of black leaf spot disease was found after BCA was applied in laboratory condition. The best BCA in laboratory are Fusarium oxysporum and Penicillium sp. Field trial on efficacy of BCA from peanut leaves was conducted at NCSRC and at Kampangsaen Campus during 1987-88. Results indicated that Bacillus subtilis was the best BCA to reduce black leaf spot where as, no significant reduction of black leaf spot obtained from the latter location.
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