The effects of past-storage osmotic conditioning treatments on viability and repair of genetic damage in pea seeds
1999
Sivritepe, H.O. | Eris, A. (Uludag Univ., Faculty of Agriculture, Bursa (Turkey). Div. of Horticulture)
Following a controlled deterioration treatment, pea seeds were subjected to osmotic conditioning treatments at 16 deg C with PEG-8000 (-0.25, -0.50 and -0.75 MPa), ABA (10E-3 M and 10E-4 M) and distilled water (H2O) for 2, 4 and 6 days. Osmotic conditioning treatments increased the germination percentage and decreased the mean germinatin time (MGT) in pea seeds. Furthermore, cytological examinations showed that chromosomal aberrations decreased during first mitotic divisions, due to induction of DNA repair mechanism with osmotic conditioning treatments. Most of the benefits of the treatments occurred during the first 2 days. However, the maximum benefit was obtained from the 4 day treatment with H2O. The results of osmotic conditioning treatments show that the critical moisture content that facilitates repair of chromosomal damage is between 34 and 38 %
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