HPLC [high pressure liquid chromatography] separation of the hyperforin/adhyperforin mixture isolated from Hypericum perforatum L.
2000
Smelcerovic, A. (Faculty of Technology, Leskovac (Yugoslavia)) | Laatsch, H.G. | Djordjevic, S.M.
The hyperforin/adhyperforin mixture isolated from Hypericum perforatum L. was separated by the HPLC method. Beside acetonitrile and water, the mobile phase used for the separation contained trifluoroacetic acid. Due to its volatility, trifluoroacetic acid is very convenient both for the analytical and preparative separation of hyperforin/adhyperforin.
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