The status of Rhizomania in Belgium
2001
Meunier, A. | Schmit, J.F. | Stas, A. | Marlier, A. | Bragard, C. (Universite catholique de Louvain, Louvain-la-Neuve (Belgium). Faculte d'Ingenierie biologique, agronomique et environnementale. Unite de Phytopathologie) | Wauters, A. | Steyer, S.
Rhizomania is an economically important disease of sugar beet caused by Beet necrotic yellow vein virus. Within the context of a study of soils in Belgium infected by this virus, a survey was carried out to detect the virus in sugar beet and soil samples using ELISA, RT-PCR and bait plant techniques. Sixty-nine soils samples, taken from locations determined by the Global Positioning System, were collected during the sugar beet season in 2000. In 18 of those soils the virus was detected using ELISA, but in a further 18 the response was ambiguous. Using the RT-PCR method, the virus was detected in 28 soils. With the bait plant technique, 26 soils were showed to be infected. Beet necrotic yellow vein virus is present in all the regions in Belgium where sugar beet is cultivated. We also tried to detect the presence of RNA-5, an RNA that amplifies the symptoms caused by the virus, in our samples but none of them gave a positive response for this fifth RNA molecule.
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