Efficient regeneration from mature leaf explants of Indian mulberry via organogenesis | Régénération efficace à partir d'explants de feuille adulte chez le mûrier indien
2001
Kapur, A. ((University of Delhi South Campus, New Delhi (Inde). Department of Plant Molecular Biology)) | Bhatnagar, S. | Khurana, P. | Eswara Rao, M.S.
Leaf explants of two elite cultivars of mulberry, namely, K-2 and DD, were cultured on various media for regeneration. The explants were obtained from field-grown plants, in vitro raised seedlings, and in vitro maintained axillary shoot cultures. The regeneration percentage was maximum (up to 60 /) from leaf explants of field-grown plants followed by the leaf explants taken from in vitro cultures (up to 40 /). The highest percent of regeneration (61.4 /) was observed on MS + IAA (2 mgl/l) + TDZ [thidiazuron] (1.1 mg/l) + AgN03 (2mg/l) in both the cultivars. TDZ played an important role in the induction of adventitious buds (up to 60 /) from the basal portion of leaf with 8-10 shoots/culture. Upon regular subculture, regeneration from the callus could be observed over a year with as many as 15-20 shoots/culture. Histological examination indicated regeneration by the organogenetic mode. Rooting was obtained in 95-100 / shoots and field survival rate was 80-85
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Institut national de la recherche agronomique