A chromatographic procedure for the determination of carotenoids and chlorophylls in vegetable products
2001
De Sio, F. | Servillo, L. | Loiuduce, R. | Laratta, B. | Castaldo, D.
Pigments, such as carotenes, xanthophylls and chlorophylls, were extracted from both vegetables and their industrial products by using a 2:1 (v/v) dichloromethane/methanol solution. To separate and quantify the components in the extraction mixture, a HPLC analysis on reversed phase C30 column and binary gradient, made of methanol/water solution and dichlormethane, was employed. This gradient appears to have some advantages over other reported methods, which utilize reversed phase C30 column, in terms of resolution and analysis time.
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