Characterization of partially purified acid phosphatase from susceptible and resistant cultivars of tomato against root knot nematode Meloidogyne incognita
2000
Ganguly, A.K. | Sreedhar, Madhvi | Swain, S.C. (Indian Agricultural Research Institute, New Delhi (India). Division of Nematology)
Investigation was carried out to characterize acid phosphatase from uninoculated and inoculated tomato cultivars Pusa Ruby (Susceptible) and Pusa Nemamukt (Resistant) against Meloidogyne incognita. The enzyme was partially purified using Sephadex G-25, Ammonium sulphate precipitation and Sephadex G-200. Highly purified enzyme with high specific activity was obtained from all the four samples. On characterization it was observed that the pH optima for the enzyme was between 3.5 and 4.5, temperature optima betwen 30§C and 40§C. The enzyme has wide substrate specificity favouring both synthetic and natural substrates, inhibited by sodium phosphate, molybdic acid and copper sulphate. There was no difference in Km value between resistant and susceptible cultivar. But Vmax was highest in the resistant inoculated cultivar.
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