Growth Characteristics of Azotobacter vinelandii UWD Using Glucose and Organic Acids as Substrates
2000
Kim Tae-Yong | Park Chang-Ho Kyung Hee University, Yongin-si (Korea Republic)
Azotobacter vinelandii UWD synthesizes poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), one of the biodegradable polymers, when odd and even number carbon sources are simultaneously added to a medium. In this study, we investigated the specific growth rate of Azotobacter vinelandii UWD on propionic acid and valeric acid. The specific growth rates were 0.183 hr deci and 0.137 hr deci at 1.0~1.5 g/L of propionic acid and 1.0 g/L of valeric acid, respectively. When a mixture of 0.75 g/L of propionic acid and 0.5 g/L of valeric acid was added to the medium, the specific growth rate was 0.196 hr deci, which was equal to or higher than those of the individual organic acids. Among 10~50 g/L of glucose cell growth was best at 20 g/L.
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