Production of elite planting materials of sentang (Azadirachta excelsa L.) via in vitro culture technique
2000
Liew, T.K. | Chan, L.K. | Teo, Chris K.H. (Sains Malaysia Univ., 11800 Penang (Malaysia). School of Biological Sciences)
Eight superior clones of Azadirachta excelsa L. were identified from 150 six months old sentang seedlings population using a programme created from the PASCAL programming language based on their height and diameter. Axillary bud of the superior clones cultured on Murashige and Skoog (MS) medium supplemented with 1.0 mg per litre BA + 0.5 mg per litre NAA produced the most number of shoots (eight shoots per explant). In vitro shoots cultured on MS medium + 0.5 mg per litre NAA + 1.0 mg per litre IBA induced 55 percent of the shoots to root. Eighty per cent of the rooted shoots survived after being acclimatised for four weeks and transplanted to the soil.
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