Varietal response of Lycopersicon esculentum L. to callogenesis and regeneration
2001
Chaudary, Z. (National Agricultural Research Centre, Islamabad Pakistan). Agriculture Biotechnology Inst.) | Feroz, I. | Ahmed, W. | Rashid, H. | Mirza, B. | Quraishi, A.
The varietal response of Feston and Nagina were tested using hypocotyl and leaf disc as explant source. The callus formation was achieved on MS medium supplemented with 2,4-Dichlorophenoxy acetic acid (2,4-D) and on MS medium supplemented with Napthalene Acetic Acid (NAA) and Benzylamino Purine (BAP) from both explant sources. Shoot formation from calli of Feston and Nagina were successfully obtained on different hormonal levels of BAP & NAA on MS medium. Maximums shoot percentages of calli derived from hypocotyf (56.00%) and leaf disc (42.30%) was achieved on MS medium with 0.05 mg/l IAA and 4.0 mg/l BAP for Feston. For Nagina, maximum shoot percentage was obtained from leaf explant (39.13%) and hypocotyl (32.00%) on similar medium. Shoot regeneration produced 3.137 and 2.353 mean number of shoots/explants for hypocotyl and leaf disc of Feston whereas Nagina responded with 2.187 and 1.783 mean number of shoots/explant for leaf disc and hypocotyl respectively.
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