Biotechnological implication of flagellate protozoans in cleaning industrial effluents containing heavy metal Cr+6
2000
Qazi, J.I. | Ashraf, U. (Punjab Univ., Lahore (Pakistan). Dept. of Zoology)
A flagellate protozoan was isolated from tanneries effluent collected from the vicmity of city Kasur. Axenic culture of the protozoans was established in 0.1% aqueous solution of molasses supplemented with antibiotic chloramphenicol. The medium supported a healthy growth of the microorganisms both in terms of number as well as size, however, these parameters decreased after one week and few days, respectively. Following Cr-6 exposure it was found that up to 4 microgram of the metal ml of the medium. The protozoans showed, but a significantly decreased growth throughout the study period. In race of higher concentrations: 6, 8 and 10 microgram of Cr+6/ml the protozoans showed a further delay of one- day in the manifestation of growth. Following 9, 7 and 6 days of incubation the protozoans were not observed in case of the culture media containing 6, 8 and 10) microgram of Cr+6/ml, respectively. Cultures of the flagellates exposed to chromium were found invariably devoid of dividing cells, throughout the observation period. The flagellates were found to reduce Cr+6, up to a level of 6 microgram/ml of the medium. Beyond this level the protozoan remained unsuccessful to reduce the metal.
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