Washing of buffalo bull semen affects conception rate under field conditions
2000
Ahmad, M. | Ahmad, K.M. | Rehman, S. | Shah, Z.A. (University of Agriculture, Faisalabad (Pakistan). Dept. of Animal Reproduction)
Twenty pooled good quality buffalo bull semen samples were used in this study. Each sample was divided into five aliquots (A to E). Aliquot A was used to serve as control. Seminal plasma was removed by centrifugation from aliquot C, D and E. whereas it was not removed after centrifugation from aliquot B. Aliquot D was washed simply with 2.9% sodium citrate solution and aliquot E twice. All treated and control aliquots were extended in milk-egg- yolk glycerol extender and were used for insemination to note effect on conception rate (CR). CR was checked 45 days after insemination. The poorest 38.27% CR was observed with semen sample B. The CRs of samples A (53.85%), C (58.90%) and D (64.20) differed non significantly (P less than 0.05) from each other. But CR of E (69.30%) differed significantly (P less than 0.05) from sample A. However CR of sample E differed non significantly with the CR of C and D. It may be concluded that removal of seminal plasma and washing (single/double) improves conception rate in buffaloes.
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