Cataloging stress-inducibe genes and pathways leading to stress tolerance
2000
Bohnert, H. (University of Arizona, Arizona (USA). Department of Biochemistry) | Fischer, R. | Shinji Kawasaki | Michalowski, C. | Hong Wang | Yale, J. | Zepeda, G. (University of Arizona, Arizona (USA))
We have chosen a molecular approach to investigate the reactions plants use to attenuate or ameliorate osmotic stress. We ask several questions of increasing complexity: (i) how many osmotic stress-responding genes might be contained in plant genomes ? ( ii) what types of genes constitute the stress response ? and ( iii) where and how strongly are these genes expressed. Because of recent technological advancements and breakthroughs in genome analyses meaningful answers to such questions have become possible (Bouchez and Hofte 1998). We have begun working on a project designed to detect the number and nature of all transcripts that respond to salinity and drought stress in higher plants. Main components of the project are mutant generation, the analysis of several non-plant models, and the large-scale determination of osmotic stress-regulated transcripts. DNA sequencing of approximately 10,000 ESTs each from two halotolerant (Mesembryanthemum, Dunaliella) and two salt-sensitive (Arabidopsis and Oryza) species is underway. The combination of transcript sequencing, microarray analysis, and bioinformatics will result in defining the set of genes for osmotic stress responses common to all plants.
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