Biochemical properties and application for wastewater treatment of Pseudomonas aeruginosa LP602 lipase
1998
Boonchuta Kuhasuntisuk | Saovanee Dharmsthiti (Mahidol Univ., Bangkok (Thailand). Inst. for Research and Development in Science and Technology. Center for Biotechnology)
Lipase from Pseudomonas aeruginosa LP602, a bacterial strain isolated from a domestic wastewater sample, was preliminarily characterized. The enzyme exhibited maximum lipolytic activity at pH 8.0 where it was also most stably maintained. At 55 dec g, the lipase had the highest activity but not stability. The enzyme was insensitive to EDTA and to many ions tested except Zn++. It was sensitive to SDS but not to Tween-20, Tween-80 or Triton X-100. The enzyme was found to be active towards a number of commercial food grade fats and oils. The suitable medium formula for lipase production was MMP containing 6.25 percent whey as a carbon source, 1 percent soybean oil as inducer and 0.5 percent yeast extract supplement. The culture had to be fed with glucose to a final concentration of 0.1 percent at the 15th hour of incubation. The lipase production under this condition was 3.5 U/ml. Both P. aeruginosa LP602 cells and lipase were shown to be usable for lipid-rich wastewater treatment.
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