Molecular cytogenetic analysis of wheat-alien hybrids and derivatives
2002
Molnar-Lang, M. | Linc, G. | Nagy, E.D. | Schneider, A. | Molnar, I.
New wheat x barley, wheat x Aegilops biuncialis and wheat x rye hybrids were produced with the aim of alien gene transfer from these species into wheat. Amphiploids were produced with the help of colchicine treatment from the last two combinations. The new wheat x barley hybrids were multiplied in tissue culture because of the high degree of sterility and then pollinated with wheat to obtained backcross progenies. Wheat-barley chromosome pairing was detected using genomic in situ hybridization (GISH) in two combinations (Mv9 kr1 x Igri, Asakazekomugi x Manas). In vitro conditions caused an increase in chromosome arm association frequency in both combinations and in fertility in some regenerants.
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