Comparison of different methods in the determination of cholesterol
2002
Sterna, V. (Latvia Univ. of Agriculture, Sigulda (Latvia). Research Centre "Sigra")
The aim of the present study was to determine the level of cholesterol in the milk of the Latvia's cow herds, analyse differences between two different methods of cholesterol determination in milk. Milk samples were obtained from the cows of Latvian Brown and Black and White breeds. The milk fat and protein were analysed by Milkoscan 133, cholesterol content was determined colorimetricaly by Blur procedure with prior lipid extraction by Roese-Gottlieb method and by gas-chromatography method. The obtained results showed that the milk fat content was 4.48+-0.28%, protein was 3.03+-0.09%, cholesterol content in milk was 21.05+-2.01 mg 100 ml*[-1), when assayed by spectrophotometer SF 26 LOMO and 18.29+-1.73 mg 100 ml*[-1), when assayed by gas chromatography. There were no significant differences in cholesterol content between both determination methods.
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