Utilization of anther culture technique in the generation of new genetic traits for adverse conditions
2003
Lapitan, V.C. | San Valentin, G.O. (Philippine Rice Research Inst., Los Banos, Laguna (Philippines))
Biotechnology can certainly provide solutions to most problems associated with breeding improved rices with tolerance to abiotic stresses. Anther culture technique offers great opportunities for accelerating breeding progress and generating variation useful for crop improvement. From a total of 34,240 anthers of IR64 subjected to anther culture, 33 regenerated 139 green plants of which only 9 survived when transfered in the screenhouse. At maturity, 88 panicles were harvested and planted in the field for evaluation. Each panicle was designated as a doubled haploid (DH) line. Based on the number of tillers, plant height, number of spikelets within a panicle, uniformity, and phenotypic acceptability, 3 DH lines (VAC5, VAC7 and VAC8) were selected. When subjected to zinc deficient condition, the 3 DH lines performed better than the seed-derived IR64. Among the 3 however, VAC 5 gave the best performance. Evaluation of their root system also showed improvement in terms of root length and weight. Using the Minus One Element Kit (MOET), VAC 8 was further exposed to different soil-related stresses. Results showed that it was more resistant to P, K, Zn, S, and Cu deficiencies than the seed-derived IR64
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