Utilization of anther culture technique for the development of inbreds for transplanted irrigated lowland rice
2003
Lapitan V.C. (Philippine Rice Research Inst., Los Banos, College, Laguna (Philippines)) Cayaban, E.B. Jr | Roferos, L.T.
Fixation of desirable traits such as high yield, good grain qualities, and resistance to pests and diseases usually takes several generations using the conventional methods. Anther culture techniques was reported to shorten the conventional cycle by 3 to 4 generations. Using the technique, elite lines were produced within 2 years from anther culture while 5 years using the conventional method. From a total of 1,229 anthers of F1-506, a cross between C4044-2B-2-2 and IR13540-56-3-2-1, which were subjected to anther culture, three anthers regenerated 11 plants of which only one survived when transferred in the screenhouse. At maturity, four panicles were harvested and planted in the field following the panicle to a row method for initial seed increase. Based on their uniformity, number of productive tillers, plant height, number of spikelets within a panicle, and phenotypic acceptability, 11 individual plants were selected and evaluated under replicated yield trial (RYT). Likewise, results of grain quality evaluation revealed that the selected doubled haploid (DH) lines were better than the parents. On the succeeding yield trials, three elite DH lines were selected as they outyielded the check varieties, IR72 and PSBRc 18, and showed stable performance. These lines will be channeled to National Cooperative Testing (NCT) next season, 2003 wet season
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