Use of molecular markers for heterotic grouping of publicly developed maize inbred lines in the Philippines
2003
Sales, E.K. (University of Southern Mindanao, Kabacan, Cotabato (Philippines) Guzman, P.S. | Magulama, E.E. | Butardo, N.G.
Ninety-three maize inbred lines developed at USM, UPLB [Philippines Univ. Los Banos, Laguna, Philippines], and IES were assayed for polymorphism at 40 SSR [simple sequence repeats] marker loci using polyacrylamide gel electrophoresis (PAGE). The objectives were: to screen a set of stable and repetitive SSR markers for DNA fingerprinting or heterotic grouping and exploit the heterotic patterns among domestic inbred lines. Two hundred six alleles were identified and served as raw data for estimating genetic similarities among these lines. A cluster analysis placed the inbred lines in six clusters that is suggestive of the major heterotic group for Philippine maize. Specific alleles exclusive for certain lines were also detected. These can be used as a unique fingerprint for such inbred and therefore might be a useful marker to distinguish a character trait exhibited by that particular line. The study confirms the utility of polymerase chain reaction (PCR) based markers such as SSRs for measuring genetic diversity, for assigning lines to heterotic groups and for genetic fingerprinting
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