Organ and tissue culture of Acacia senegal (L.) willd
2001
El-Tigani, S. (University of Khartoum, Khartoum (Sudan). Faculty of Science, Dept. of Botany) | Ali, Y.H. (Forestry Research Centre, Soba, Khartoum (Sudan)
In vitro shoot multiplication and callus culture of Acacia senegal (L.) Willd. have been demonstrated. Explants were taken from apical and axillary shoots (young and mature). Murashinge and Skoog basal medium containing 1-naphthalene acetic acid (NAA), N**6-benzyl aminopurine (BAP) and kinetin at 0.5-2.5 mg/1 each was used. Shoot regeneration was obtained but the multiplication rate was low (x1 and x2). Also, the shoots regenerated showed a high mortality rate due to the small number of shoots produced per explant. Explants of different ages and origins were used for callus initiation. The callus was formed at the basal cut end of the explant with varying intensities and coloration with the various concentrations of NAA and Kinetin used at 0.5-2.5 mg/1. Subsequent sub-cultures showed a high capability of A. senegal callus tissue for differentiation and development. Ovulation, proembryos, endospermic cells and whole mounts of mature embryos were clearly demonstrated. Plant tissue was maintained in culture for up to 12 months through subcultures every four weeks without apparent loss of viability. Also, juvenile tissue was found generally more responsive in culture compared to mature tissue
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