The NUD1 gene of yeast Saccharomyces cerevisiae complements a thermosensitive lytic mutation
2003
Alexandar, I. (Institute of Molecular Biology - Bulgarian Academy of Sciences, Sofia (Bulgaria)) | Vazquez de Aldana, C. | del Rey, F.
Temperature sensitive (ts) yeast strains have been obtained by ethyl methane sulfonate (EMS) mutagenesis. They have a normal growth at low temperature releases of intracellular proteins in the culture medium ot 37 degree C. The ts mutation was localized in the NUD1 gene, which codes for a component of the yeast spindle pole body (SPB). Construction of a frame shift mutation in the wild type gene demonstrated that the correct sequence of the NUD1 gene only could complement the ts phenotype. Further analysis showed that the ts mutation deprived the cell of a normal termination of mitosis, thus causing death at restrictive temperature and a consequence cell lysis.
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