Developing genetic variability by somacloning in sunflower (Helianthus annuus L.) and it's application for improvement
2004
Encheva, J. | Tsvetkova, F. | Ivanov, P. | Todorova, M. | Shindrova, P.
Immature zygotic embryos from the Spanish fertility line Z-8-A were used as donor material for induction of direct organogenesis in sunflower (Helianthus annuus L.). The range of spontaneous somaclonal variation among the progenies of regenerants was studied. The genetic modifications observed in the regenerants included twelve agronomic traits. Somaclonal lines R 1305, R 1279 and R 1303 showed a modified architecture, higher oil content in seed and higher 1000 seed weight in comparison to the standard Z-8-A, and very good combinaing ability. Hybrid 117, 126, 128 produced with the participation of somaclonal lines R 1279, R 1303 and R 1305 demonstrated high production capacity shorter vegetation period and reduced height than comercial standards Albena and Super Start. The combination of this favourable changes is desirable in breeding work on sunflower. Somaclonal variation through direct organogenesis has fasilitated the creation of genetically heritable variation in sunflower, which can be used with success for hybrid seed production of highly productive and adaptible hybrids.
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