Laparoscopic and non-surgical transfer of fresh and frozen porcine embryos
2003
Riha, J. (Vyzkumny Ustav pro Chov Skotu, Rapotin (Czech Republic)) | Vejnar, J.
The aim of this research was to evaluate the impact of different factors on the efficacy of embryo transfer in swine. Embryos in the stage of expanded, hatching and hatched blastocyst were recovered post mortem from superovulated donors. Synchronised recepients showing standing reflex at the time of donor insemination were used for laparoscopic and non-surgical ET on day 5.5-6.0 of the cycle. In total, 36 ET were evaluated, 19 recipients were pregnant, 4 of them aborted. No significant differences were found between gilts and sows in success rates of the laparoscopic transfer of fresh and frozen embryos, differences in birth rate were statistically significant in some experimental groups. ET of fresh embryos and ET of vitrified embryos resulted in 48 % (12/25) and 63.6 % (7/11) pregnancy rate, resp. (P0.05). The birth rate resulting from vitrified embryo transfers was significantly higher (P0.01), but on average a higher number of thawed embryos was transferred (10.8+/-2.10 fresh and 13.1+/-4.22 thawed embryos per pregnant recipient). Pregnancy rate in recipients of non-surgically transferred embryos ranged from 25 to 50 %. The rate of surviving embryos out of transferred embryos ranged from 36.1 to 69.2 % and amounted to approximately 50 %, the rate of weaned piglets oscillated at 95 % level in all experimental groups.
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