Analysis of genetic variation of eight candidate genes in two wild boar subspecies
2003
Ernst, M. | Kuciel, J. | Urban, T. (Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic). Ustav Genetiky)
Genetic variability and diversity of two subspecies of wild boar, Sus scrofa scrofa (SSS, n=67) and Sus scrofa attila (SSA, n=42) were studied by the PCR-RFLP method. Eight candidate genes with two-allele polymorphisms were analysed. No polymorphism in genes encoding ryanodine (RYR1=CRC) and oestrogen receptors (ESR) was found in either of the subspecies. In SSS, the frequencies of alleles of individual genes were as follows: MYC protooncogene protein (MYC) A=0.9; growth hormone (locus GH-HaeII)+=0.57 and (locus GH-MspI)+=0.43; leptin (LEP) T=0.79; heart fatty acid-binding protein (H-FABP) H=1.00; prolactin receptor (PRLR) A=0.22 and follicle-stimulating hormone - beta polypeptide (FSHB) A=0.40. In SSS, average heterozygosity of genotypes was 0.295. The SSA subspecies showed the following allele frequencies of individual genes: MYC A=0.01; GH-HaeII+=0.65 and GH-MspI+=0.35; LEP T=1.00; H-FABP H=0.98; PRLR A=0.33; FSHB A=0.49. In SSA, the average heterozygosity of genotypes was 0.216. Highly significant differences in genotype frequencies between these two subspecies were found in MYC and LEP. PIC values of the polymorphic loci ranged from 0.023 to 0.375 in both subspecies. Differences between both subspecies of wild boar on the one hand and in comparison with some breeds of domestic pigs were found in the polymorphism of genes RYR1, ESR, and MYC.
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