Pathogenesis of murine lymphotropic gammaherpesvirus isolate 78
2003
Mrmusova-Supolikova, M. | Pappova, M. | Mistrikova, J. (Univerzita Komenskeho, Bratislava (Slovak Republic). Katedra Mikrobiologie a Virologie)
BALB/c mice were infected intranasally with the mouse lymphotropic gammaherpesvirus isolate 78 (MHV-78). Infectious virus was detected in various organs of infected mice at different time intervals during a period of 180 days post infection (p.i.) by cocultivation with permissive VERO cells and indirect immunofluorescence using monoclonal antibodies prepared against MHV. We observed the highest infectious titer of MHV-78 during the acute infection on day 15 in lungs, and on days 7 and 15 in blood. At later intervals of infection the virus was detected in spleen, peritoneal macrophages, thymus, bone marrow and mammary glands. The infection with MHV-78 was accompanied with the changes in the number of different leukocytes associated with the appearance of atypical leukocytes. In acute infection the frequency of atypical leukocytes reached its maximum at day 15 p.i., while in the course of persistent infection it was at day 122 p.i. Approximately 7 % of infected BALB/c mice developed tumors. Cells derived from tumor were positive for viral antigen. These results showed that MHV-78 is lymphotropic and oncogenic.
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