Multiple shoot induction and plant regeneration from embryo axes of six cultivars of Gossypium hirsutum
2003
Banerjee, A.K. (Iowa State Univ., Ames (USA). Horticulture Hall) | Agrawal, D.C. | Nalawade, S.M. | Hazra, S. | Krishnamurthy, K.V.
The report describes in vitro plant regeneration from embryo axis explants of six cultivars of cotton. Induction of a maximum number of multiple shoots in all six cultivars could be achieved on Murashige and Skoog salts and Gamborg (B5) vitamins supplemented with 0.4 microM benzyladenine and 0.1 microM napthaleneacetic acid (NAA). Elongated shoots could be rooted on half strength medium supplemented with 0.5 microM NAA. Rooted shoots survived (92 %) after hardening in the greenhouse and grew to maturity (100 %) after transfer to field.
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