Decolorization of orange II by Penicillium sp. and fermentation of intermediate product aromatic amina
2000
Wiloso, E.I. | Sumartini, S. (Pusat Penelitian dan Pengembangan Bioteknologi, Cibinong, Bogor (Indonesia))
English. Decolorization process of synthetic wastewater containing orange II by Penicillium sp. in aerobic conditions was observed by using spectrophotometer, and the formation of aromatic amine as the intermediate product of the azo degradation was detected by gas chromatography. The experiments were carried out in a 500-ml flask with media containing 3000 mg/l glycerol as the main carbon source and 100 mg/l Orange II as the colored pollutant. After 7 days of incubation, color removal rate than strain L2K. The formation of an intermediate product, which was predicted as naphtyl amine, was observed during decolorization process, but it did not accumulate as concentrations were decreasing to a negligible level after 6 days of incubation
Show more [+] Less [-]Indonesian. Proses dekolorisasi limbah cair sintetik yang mengandung orange II oleh Penicillium sp. pada kondisi aerobik diamati dengan spektrofotometer dan pembentukan amina aromatik sebagai senyawa-antara hasil penguraian zat warna tersebut diikuti dengan kromatografi gas. Percobaan dilakukan dalam labu 500 ml dengan media yang mengandung gliserol sebagai sumber karbon utama sebanyak 3000 mg/l dan orange II sebagai bahan pencemar sebanyak 100 mg/l. Pengamatan selama 7 hari masa inkubasi menunjukkan bahwa Penicillium sp. dapat menurunkan konsentrasi orange II hingga 99 persen, galur L2A mempunyai kemampuan penghilangan warna yang lebih cepat daripada galur L2K. Keberadaan senyawa amina aromatik, hasil penguraian orange II, yang diperkirakan sebagai naftilamina tidak bersifat kumulatif karena pada hari keenam masa inkubasi jumlahnya berkurang hingga dapat diabaikan
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