Suitability of gelatin for preservation of in vitro propagated theileria annulata schizonts
2000
Khatri, N. | Nichani, A.K. | Sharma, R.D. | Khatri, M. (Chaudhary Charan Singh Haryana Agricultural University, Hisar (India). College of Veterinary Science).
The protozoa Theileria annulata causes an important disease, bovine tropical theiloriosis in cattle which is transmitted by the ticks of the genus Hyalomma. Exotic cattle, their crossbreds and young indigenous calves are highly suspectible, whereas adult indigenous cattle are relatively resistant. To minimize economic losses due to this disease, macroschizont stage of the parasite has been propagated in vitro, attenuated by continuous passaging and used as a cell culture vaccine in many countries including India for control of this disease. The cell culture vaccine has to be cryopreserved and transported in liquid nitrogen which is a very costly affair. The present experiments were conducted to study the suitability of gelatin in preservation of in vitro propagated Theileria annulata (Hisar) schizonts at 4 degree celcius.
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