Visible and Fast Assay System for Tobacco Transformant Introduced with Adenosine Deaminase Marker Gene
2001
Yang, D.C. (Korea Gineng and Tobacco Research Institute, Taejon, Korea Republic) | Kim, Y.H. (National Institute of Agricultural Science and Technology, Suwon, Korea Republic)
New visible and fast assay system have been developed for tobacco transformant introduced with adenosine deaminase (ADA) marker gene, which converts cytotoxic adenosine analogues to non-toxic inosine analogues and ammonia. Ammonia was changed to blue color in the solution of phenol-nitoprusside and alkaline-hypochlorite. It was possible to detect activity of ADA visibly on the holes of 96 well plate using tiny explant of transgenic tobacco leaves within 1 hour incubation time.
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