Ion-Sensitive Field Effect Transistor-Based Multienzyme Sensor for Alternative Detection of Mercury Ions, Cyanide, and Pesticide
2003
Volotovskky, Vyacheslava (Institute of Molecular Biology and Genetics, Kiev, Ukraine) | Kim, N.S. (Korea Food Research Institute, Songnam, Republic of Korea)
Various groups of industrial and agricultural pollutants can be detected by enzymes. Since heavy metal ions inhibit urease, cyanides inhibit peroxidase, organophosphorus and carbamate pesticides inhibit butyrylcholinesterase, these enzymes were co-immobilized into a bovin serum albumin gel on the surface of an ion-sensitive field effect transistor to create a bioprobe that is sensitive to the compounds mentioned above. The sensitivity of the present sensor towards KCN corresponded to 1mM with 1 min of incubation time
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