Storage of the seeds of indigenous orchids
2003
Sotto, R.S. (Philippines Univ. Los Banos, College, Laguna (Philippines))
The author devised a modified procedure in determining the moisture content (MC) of orchid seeds and established a quick biochemical method to estimate the viability of orchid seeds in the study, "Conservation of diversity of indigenous orchid species using seeds in fruit capsules" under the project "Investigation into the conservation of biodiversity of indigenous orchids using seed", from 1998 to 2001. The first procedure used 20 mg of duplicate samples (weighed to four decimal places) dried at 130 deg C for 30 minutes. To estimate the viability of orchid seeds , the researcher used the topographical tetrazolium test (TTZ) which was modified: 4-5 hours incubation at ambient temperature, 1% TTZ, and pink embryos considered as viable. Seeds of 265 accessions were dried in glass bottles with silica gel and placed in the drying room. Seeds were stored at 0 deg C for an interval of three months (131 accessions), less than 24 months (245 accessions), and 24 months and above (29 accessions). Of these, 70 accessions were tested for viability after a year of storage and eight accessions were tested after two years. Phalaenopsis amabilis and Geodorum sp. had the highest seed viability, 95.5% and 86.67%, respectively. The viability of orchid seeds tested after a year in storage generally decreased. After two years of storage, seed viability varied. The viability of seeds of Renanthera monachica decreased at 2%, while Dendrobium heterocarpum decreased at 65%
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by University of the Philippines at Los Baños