Application of DNA molecular markers to maize improvement in the Philippines
2003
Sales, E.K. (University of Southern Mindanao, Kabacan, Cotabato (Philippines). Dept. of Plant Breeding and Genetics) Guzman, P.S. | Manceras, A.D. | Magulama, E.E. | Salazar, A.M.
The Molecular Biology Laboratory at USM [University of Southern Mindanao, Kabacan, Cotabato, Philippines] was established through the DA-BAR [Department of Agriculture - Bureau of Agricultural Research, Diliman, Quezon City, Philippines] IDG Fund in partnership with AMBIONET. This brings new technology to USM in reaching its goal of state-of-the art research. This project aims to: enhance the capability of public maize breeding institutions in the Philippines through biotechnology tools and develop maize germplasm through biotechnology for resource to assess the genetic diversity of publicity developed inbred lines in the country; develop quality protein maize (QPM) hybrids adapted in the Philippines; transfer the QPM allele (opaque 2) to an elite Philippine inbred through marker assisted backcrossing; contribute to the CIMMYT [Centro Internacional de Mejoramiento de Maiz y Trigo, Mexico] led drought tolerance mapping project by evaluating RILS under drought conditions. Training of USMARC research staff was conducted at USMARC Genetics and Molecular Biology Laboratory through AMBIONET for familiarization with different equipment and their uses. Likewise, lectures and demonstrations on DNA extraction, RNase treatment, DNA reprecipitation, DNA resuspension, agarose gel electrophoresis, Simple Sequence Repeats - Polymerase Chain Reaction (SSR-PCR), Denaturing Polyacrylamide Gel Electrophoresis (PAGE), Silver Staining, Scoring DNA bands and Data Analysis were also conducted. Diversity Analysis of 43 inbred lines from UPLB, 11 from Ilagan Experiment Station (IES) and 45 from USMARC, USM inbred lines is ongoing. Screening of CIMMYT drought parental lines and field evaluation of F1 segregating population will be done. Moreover, development of QPM cultivars and molecular assisted (MAS) introgression of opaque allele to an elite Philippine inbred line will be done. So far, 55 CML's and 5 QPM lines from CIMMYT Mexico have just arrived and are ready for planting. Likewise, incorporation of opaque 2 gene to a Philippine DMR line will also be done by using 2 markers in the backcrossing work
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