Construction of a suitable vector for lacz gene expression in Hansunela polymorpha
2003
Rezaee, A. (Tarbiat Modarres Univ., Tehran (Iran). Dept. of Medical Micribiology)
In the present study Hansunela polymorpha plasmid was constructed to express lacZ gene under yeast gene promoters. Hpleu2 gene was used as marker and to target the integration of plasmids into the corresponding Hansunela polymorpha genome locus. LacZ and Hpleu2 genes insert into polylinker site of pBluscript 11 ks. Expression was obtained with regulatable MOX promoter. Utility of the vector was illustrated by expressing the bacterial lacZ gene. The vector constructed are potentially useful for the construction of efficient producers of heterologous proteins in Hansunela polymorpha.
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