Effects of E20 and Ms based media on in vitro induction of axillary buds and shoot development from haploid Cucumis melo microcuttings
2003
Bal, U. (Trakya Univ., Tekirdag (Turkey). Dept. of Horticulture) | Sari, N. | Yilmaz, H.
The culture medium E20A was used as part of the dihaploidization technique in the genetic improvement of Cucumis melo. During long term maintenance of haploid stock plants via subculture of microcuttings, weak shoot development and death of plantlets were observed. Therefore in search of a suitable medium to induce vigorous development of the long term maintained plantlets, the present experiment was conducted. The basic media E20A and MS were used with or without only one concentration of IAA, i.e., 0.01 mg 1-1, in combination with one of the three concentrations of BAP, i.e., 0.225 mg 1-1, 1.125 mg 1-1 and 2.250 mg 1-1. Plantlet length, number of nodiums, callus length and diameter, rates of root and shoot initiation were recorded weekly during culture. It was determined that while single shooting and up-to-the-standard plantlets were obtained using E20A medium containing 0.01 mg 1-1 IAA (medium one), multi shooting plantlets with shorter intemodiums were obtained from the E20A medium only with 0.225 mg 1-1 BAP (medium three). The most desirable shoot development was obtained from the media without BAP but containing 0.01 mg l-1 IAA. Integration of BAP into the media resulted in callus development which in turn restricted shoot development. It was concluded that low salt media is more suitable for in vitro induction of axillare buds and shoot development in melon.
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