Immobilization of a fungal carboxymethylcellulases by gel entrapment and its impact on operational stability
2004
Jabbar, A. (Government Coll. Univ., Faisalabad (Pakistan). Dept. of Chemistry) | Rashid, M.H. | Malana, M.A. | Amanullah | Saleem, M. | Niaz, M. | Yasin, M.Z.
Slightly acidic form of purified CM Cases (13.97 mumg-1 protein) was immobilized in 13.6 % (w/v) acrylamide in the presence of cross linker Bisacrylamide which is 2% of the monomer acrylamide, 0.02 ml TEMED and 0.01g ammonium per sulfate per ml was added to reaction mixture which initiated polymerization. 71.34% protein was immobilized while 28.34 remain unbound. The polymerized gel containing CM Cases was cut and sliced into 1x1 mm fragments. It is stored at 4 degree C. its operational and recycling activity was observed for consecutive four operations. Enzymes were heated in a solution two hours each times. Immobilized CM Cases showed Vmax (49 mumol mg-1 mL-1), Km (4.54 mg mL-1), and specificity constant (Vmax/Km) 10.78 in its fourth operation and consecutive application.
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