Use of seed coat peroxidase test and seed urease test for varietal identification in soybean
1993
Wanchai Chanprasert | Permpong Sriprasertsak | Wanruedee Sributroj (Kasetsart Univ., Bangkok (Thailand). Faculty of Agriculture. Dept. of Agronomy)
Several varietal tests for characterising 18 soybean lines described by Wagner and McDonald (1982) which include seed coat peroxidase test, electrophoresis of urease in the unimbibed seed, hilum colour and hypocotyl colour were used in this study. These tests separated the 18 lines into 13 groups. Ten of these groups were exclusively isolated, i.e. SK1, 8122-7, KUSL20004, CM60, KUSL10008, KUSL10002, KUSL20010, KUSL20018, KUSL10009 and KUSL10001. Two to three lines remained in anyone of 3 groups. The use of these laboratory tests was found more effective than field plot test which separated only 10 groups. The soybean lines which were not separated by the tests used in this study were SJ4 and CM001-1, and KUSL20043 and KUSL 20050.
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