Somatic embryogenesis and plant regeneration in passion fruit hybrid (Passiflora edulis Sims. P. edulis f. flavicarpa)
1993
Ariya Satrabhandhu | Angkab Punyashthiti | Kitti Wattanasermkit (Royal Chitralada School, Bangkok (Thailand). Plant Tissue Culture Laboratory)
Plants were regenerated from callus derived from epicotyls, cotyledons, and hypocotyls of Passiflora edulis Sims. x P. edulis f. flavicarpa via somatic embryogenesis. The frequency of somatic embryo formation for epicotyls, cotyledons, hypocotyls and roots was 63 percent, 17 percent, 89 percent and 0 percent respectively. Presoaking of explants in liquid MS medium containing a high concentration of BA was essential to achieve the rapid somatic embryo formation. The highest frequency of somatic embryogenesis occurred with hypocotyls presoaked in 5.0 mg/lt BA and cultured subsequently on the modified MS medium containing 0.25 mg/lt BA. The somatic embryos were developed on the medium containing 4 percent glucose and 15 percent coconut water. Rooting was promoted by culturing on the medium containing 4 percent sucrose and 15 percent coconut water. Plants were uprooted and grown in sterilized vermiculite in pots and subsequently transferred to commercial soil mixture. The survival rate was 90 percent.
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