Effects of cryoprotectant levels on the posthaw spermatozoa motility of frozen canine semen
1999
Mongkol Prongcharoen (Khon Kaen Univ., Khon Kaen (Thailand). Faculty of Veterinary Medicine. Dept. of Surgery and Theriogenology)
Fifteen ejaculations from three Thai dogs were collected to make frozen semen. Twenty million motile spermatozoa from each ejaculation was divided into extender containing glycera(G) and ethylene glycol(EG) at 2, 4, 6, and 8 percent (V/V). Semen was cooled from 35 deg C to 5 deg C within 35-40 min. and hold at 5 deg C for 4 hours and then kept into liquid nitrogen (-196 deg C) for two days. Frozen canine semen was thawed in warm water at 37 deg C for 1 min. Posthawed sperm motility (56.78, 52.14, 56.07 and 54.28 percent, respectively, at 0 hr). in extender containing EG4, EG2, G8 or G6 percent was not significantly different (P0.05). Sperm motility after 1 hour incubation in extender containing EG2, EG4, G6 or G8 percent was not different (21.78, 17.00, 19.06 and 17.50 percent, respectively). After three hours, sperm motility in extender containing EG2 percent (11.57 percent) and G6 percent (11.28 percent) were higher than EG4, EG6, EG8, G2, G4, G8 percent. The experiment showed that using EG2 and EG4 percent as extender to make frozen canine semen were not difference in comparison to using G6 and G8 percent. However, extender containing EG2 and G6 percent gave highest (posthawed) sperm motility after three hours incubation at 37 deg C.
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