Factors affecting root formation from leaf-derived shoots of mangosteen
1997
Te-chato, S. | Sujaree, R. (Prince of Songkla Univ., Songkhla (Thailand). Faculty of Natural Resources. Dept. of Plant Science)
Various sizes of young shoots of mangosteen derived from cultured leaves in vitro were prepared by various treatments. The treatments comprised wounding and not wounding basal part of the shoots and dipping and not dipping in a solution of indolebutyric acid (IBA). The shoots were then transferred to rooting medium solidified with agar-agar or Gelrite in the absence or presence of phloroglucinol (PG) at concentration 5.6 mg/l. The cultures were maintained in the dark for two weeks. The results showed that wounding and dipping were necessary to induce a high frequency of root formation. PG alone in the medium without any treatments could not induce root. A number of wounds applied to base of shoots showed no correlation with a number of roots produced. However, two or more wounds tended to provide the higher percent of 2-3 roots. A big sizes of shoots gave the highest rooting percentage of two roots (80 percent), followed by medium size (30 percent) and small size (5 percent), respectively. Two different types of agar provided similar percentages of rooting, but the percentage of 2 roots obtained in agar-agar solidified culture medium was far higher than those obtained in Gelrite.
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