Effect of colchicine applied to callus of mangosteen on morphological changes of regenerated plants
2000
Te-chato, S. | Sujaree, R. (Prince of Songkla Univ., Songkhla (Thailand). Faculty of Natural Resources. Dept. of Plant Science)
Calli of mangosteen raised on callus induction medium (CIM) at 20 days of culture were submerged in various concentrations of colchicine. The cultures were incubated on a rotary shaker and shaken at 80 rpm for 2 hours. In the case of high concentrations of colchicine (500 and 750 mg/l), the calli were cultured directly on the colchicine-containing media for 30 days. The results showed that leaves of regenerated shoots contained more chlorophyll b than those of control. However, shoot size, leaf area and leaf number tended to decrease while number and length of roots increased. A high concentration of colchicine, 500 mg/l, produced shoots with brown-callused leaves. However, roots could be induced from these shoots to form complete plantlets. Some of the intact leaves produced a large number of shoots. Colchicine at 750 mg/l gave some morphological abnormalities with 5 roots per shoot. A small shoot could produce a large healthy root. Some shoots produced 3 leaves per whorl.
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