Acid tolerance and antibiotic resistance of some strains of Bradyrhizobium applied in Thailand
1995
Teaumroong, N. | Murooka, Y. | Boonkerd, N. (Suranaree University of Technology, Nakhon Ratchasima (Thailand). Inst. of Agricultural Technology. School of Biotechnology)
pH sensitivity and antibiotic resistance of 20 strains of Bradyrhizobium spp. and 16 strains of B. japonicum were characterized. The characteristics of acid tolerance among Bradyrhizobium can be observed after being cultured in a defined medium for 10 days of growth. One mechanism which provides the ability to tolerate low pH conditions is alkali producing, such as Bradyrhizobium spp. strain TAL 305 isolated from Vigna radiata. The antibiotic resistance profiles when applied with Carbenicillin (500 micro g/ml), Chloramphenical (500 micro g/ml), Streptomycin (100 micro g/ml), Tetracycline (100 micro g/ml) and Trimethoprim (50 micro g/ml) were established. To differentiate them by using antibiotic resistance property, the results showed that this can be a rapid and efficient method for phenotypically distinguishing strains of Bradyrhizobium. The results obtained from this study will be combined with the data from primers-based technique.
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