Growth, water content, and proline accumulation in drought-stressed callus of date palm
Al-Khayri, J.M.(College of Agriculture and Food Sciences, Al-Hassa (Saudi Arabia). Date Palm Research Center)E-mail:[email protected] | Al-Bahrany, A.M.(College of Agriculture and Food Sciences, Al-Hassa (Saudi Arabia). Dept. of Horticulture)
This study was conducted to examine the response of date palm (Phoenix dactylifera cvs. Barhee and Hillali) calli to water stress. Callus derived from shoot tip explants was inoculated in liquid Murashige and Skoog's medium containing 10 mg/cubic dm alpha-naphthaleneacetic acid, 1.5 mg/cubic dm 2-isopentenyladenine, and 0 to 30% (m/v) polyethylene glycol (PEG 8000) to examine the effect of water stress. After 2 weeks, callus growth, water content, and proline accumulation were assessed. Increasing water stress caused a progressive reduction in growth as expressed in callus fresh mass, relative growth rate, and index of tolerance. Both genotypes tested followed this general trend, however, cv. Barhee was more tolerant to drought stress than cv. Hillali. Increasing PEG concentration was also associated with a progressive reduction in water concentration and increased concentration of endogenous free proline.
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