Contamination of meat stored in home refrigerators in Qena (Egypt)
Farghaly, R.M.(South Valley Univ., Qena (Egypt). Dept. of Food Hygiene and Control) | Gherbawy, A.M.H.(South Valley Univ., Qena (Egypt). Dept. of Botany)E-mail:[email protected] | Yosef, M.S.(South Valley Univ., Sohag (Egypt). Dept. of Botany)
Eighty samples were collected from different parts of home-refrigerators and meat stored herein, in the province of Qena, Egypt. Quantitative and qualitative estimations of moulds were carried out by conventional methods and the identified Aspergillus spp. were confirmed by the RAPD-PCR technique. The obtained results revealed that the highest mould count was 390,000 CFU/square cm in the chest of the refrigerators, followed by 320,000, 26,000 and 25,000 CFU/square cm in samples of air and freezer of refrigerators and stored meat, respectively. Eleven mould genera could be identified, the most common of which were Aspergillus, Penicillium and Cladosporium. The counts and relative frequencies for these genera were 25.4%, 13.9% and 13.1%, respectively. Five Aspergillus species were identified; mainly A. flavus (42.0%), A. niger (16.1%) and A. nidulans (16.1%). The isolated Aspergillus species were subjected to further identification by random amplified polymorphic DNA (RAPD) by using type strains from IAM. RAPD-analysis indicated that the Aspergillus strains isolated during this study were completely identical with the corresponding type strains from IAM.
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