Evaluation of functional diversity of soil microbial communities - a case study
Hofman, J.(Masarykova Univ., Brno (Czech Republic). Prirodovedecka Fakulta)E-mail:[email protected] | Svihalek, J.(Masarykova Univ., Brno (Czech Republic). Prirodovedecka Fakulta) | Holoubek, I.(Masarykova Univ., Brno (Czech Republic). Prirodovedecka Fakulta)
In our case study, we measured the functional diversity of the microbial communities of twelve soils from the small natural area to assess if this assay is suitable for routine monitoring of soil biological quality. We used the BIOLOG system and found that it was suitable for routine monitoring of soils, because it is quick and simple. Nevertheless, it is difficult to select the appropriate analysis of the BIOLOG multivariate data and to choose the best parameter which can be derived from the assay. The different analyses of the data were examined and various parameters derived from the BIOLOG assay were comparatively used to contribute to the discussion of how the data should be evaluated. We showed that not-normalized raw absorbances or trapezoid areas should be used for calculation of diversity index if the inoculum was standardized. There was no single answer to what parameter provided more correct results in the multivariate statistical analysis. Evaluating at least one not-normalized (e.g. trapezoid area) and one normalized parameter (e.g. absorbances read in fixed AWCD) was strongly suggested keeping in mind that they reveal different information.
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